核苷磷酸化酶 来源于微生物
Purine-nucleoside phosphorylase
CAS: 9030-21-1
Molecular Formula: C10H12N4O4
核苷磷酸化酶 来源于微生物 - Names and Identifiers
核苷磷酸化酶 来源于微生物 - Physico-chemical Properties
| Molecular Formula | C10H12N4O4 |
| Molar Mass | 252.22668 |
| Appearance | White freeze-dried powder. |
| Storage Condition | -20°C |
| MDL | MFCD00131739 |
| Use | It can decompose nucleosides into bases and pentose-1-phosphate, and the reaction is reversible. Purine nucleoside phosphorylase is very important in purine nucleotide remediation anabolism. The lack of purine nucleoside phosphorylase in organisms will lead to damage to T cell function. |
核苷磷酸化酶 来源于微生物 - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| HS Code | 35079090 |
核苷磷酸化酶 来源于微生物 - Vitality
100～200units/mg protein
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - Definition of enzyme activity
One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37℃.
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - Isoelectric point
PH 6.0
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - Inhibitors
Ag+，Hg2+
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - Thermal stability
30～70℃
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - PH stability
5.0～11.0
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - Method of use
When measuring the activity, the powder was dissolved in 50mM,PH7.7 potassium phosphate buffer solution. Most of the bacterial PNP is hexamer, the molecular weight of the subunit is 25kDa, the substrate specificity is not strong, and can accept adenosine, guanosine and inosine as substrates; mammals and some microorganisms (Bacillus cereus, Bacillus stearrothmolophushth 6-2)PNP is a trimer, subunit molecular weight of 30~32kDa, usually only accept inosine, guanosine as substrate; Some microorganisms, such as Escherichia coli, bacillus subtilis, Bacillus stearothermophilus, there will be two kinds of polymer PNP.
Last Update：2023-08-16 21:32:38
核苷磷酸化酶 来源于微生物 - Reference Information
| Application | Purine nucleoside phosphorylase is abbreviated as PNP or PNPase, which is one of the key enzymes in purine remedial synthesis and synthesis pathways and is widely found in prokaryotes and eukaryotes. |
Last Update：2024-04-09 21:53:21
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View History
核苷磷酸化酶 来源于微生物
Bis(tributylammonium) pyrophosphate
2-甲基恶唑-5-羧酸
邻氨基对硝基苯甲酸
3-FORMYL-2-METHOXYPHENYLBORONIC ACID
ammoniumferrocyanide[(nh4)4(fe(cn)6)]
Chromate(2-), [3-[(5-chloro-2-hydroxyphenyl) azo]-4-hydroxy-6-[(1-oxo-2-propenyl)amino]-2 -naphthalenesulfonato(3-)][1-[(2-hydroxy-4-nitropheny l)azo]-2-naphthalenolato(2-)]-, dihydrogen
Bis(tributylammonium) pyrophosphate
2-甲基恶唑-5-羧酸
邻氨基对硝基苯甲酸
3-FORMYL-2-METHOXYPHENYLBORONIC ACID
ammoniumferrocyanide[(nh4)4(fe(cn)6)]
Chromate(2-), [3-[(5-chloro-2-hydroxyphenyl) azo]-4-hydroxy-6-[(1-oxo-2-propenyl)amino]-2 -naphthalenesulfonato(3-)][1-[(2-hydroxy-4-nitropheny l)azo]-2-naphthalenolato(2-)]-, dihydrogen